WebNov 25, 2024 · Culture and differentiation of human pre-adipocytes in CHUB-S7 for discovery and validation experiments. Human preadipocyte cell line Chub-S7 were cultured and differentiated as previously described [6, 11)). The respective DMEM/F12 growth media for each condition was changed every 48 hours until 100% confluence was achieved. WebChub-S7 cells and primary cultures of human sc preadipocytes differentiate in chemical defined media (14 d) and acquire a mature adipocyte phenotype with clearly visible lipid droplets.
Characteristics of differentiated Chub-S7 cells. (a) Triglyceride ...
WebAug 22, 2003 · This cell line considered as immortal was named Chub-S7. Chub-S7 cells expressed HPV-E7 gene as detected by reverse transcription-polymeral chain reaction (RT–PCR) (data not shown). WebJul 1, 2013 · The non-differentiated and differentiated control Chub-S7 cells presented similar rate of routine respiration (33.65 vs. 33.96 pmol/s × ml, respectively). The … how a duvet works
Reconstitution of telomerase activity combined with HPV …
WebNov 1, 2013 · The Chub-S7 cell line (Nestlé Research Centre, Lausanne, Switzerland) was derived from human subcutaneous adipose tissue by coexpression of human telomerase reverse transcriptase and papillomavirus E7 oncoprotein (HPV-E7) genes ( 14) and has previously been characterized in detail showing expression of typical white adipose … WebOct 11, 2016 · Objective: To investigate the role of B12 and folic acid in the development of adipocyte dysfunction. Methods and Results: Microarray analysis of human adipocytes (CHUB-S7 cell line) cultured and differentiated in customised media with varying concentrations of B12 and folic acid led to the identification of two important pathways: … WebOct 20, 2024 · Chub-s7 cells were cultured until 24h post-confluence and differentiation was induced by adding 1mM dexamethasone, 0.5mM IBMX, 10mg/ml of insulin and 10mM of rosiglitazone to the medium for 14-21 days, until the appearance of adipocyte morphology. For quantitative real-time PCR experiments, differentiated chub-s7 adipocytes were … how advanced is rosetta stone