Tsingke tse005 2×t5 super pcr mix colony
WebNov 1, 2024 · The reaction mixture was incubated for 60 min at 42°C and stored at −20°C until required. Single RT‐PCR. Single RT‐PCR was performed in a 25 µl reaction mixture containing 1·5 µl of cDNA, 22·5 µl of 1·1× T3 Super PCR Mix (Tsingke, Beijing, China) and 0·5 µl of forward and reverse primer each (20 µmol l −1 stock). Web2 O 12.5 µL 1 µL 1 µL 见标注 Up to 25 µL 25 µL 2 µL 2 µL 见标注 Up to 50 µL 1× 0.4 µM 0.4 µM · 请使用高质量模板。 · 请勿使用dUTP和含有尿嘧啶的引物与模板。 · PCR Mix应避免反复冻融，短期内多次使用可置于2~8℃保存。使用前， Mix解冻后应充分混匀。 注意事项 .推 …
Tsingke tse005 2×t5 super pcr mix colony
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WebThermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. … Web2 ×T5 Super PCR Mix (Colony) 5. Initial denaturation. 98. 30s. 1 × . Forward primer . 0.2. Denaturation. 98. 10s. 29 × . Feverse primer . 0.2. Annealing. T m-5. 30s. Template. 2. …
WebA 2× T5 Super PCR Mix (Colony) (cat. no. TSE005, Tsingke, Beijing, China) was used to identify the genes of single bacteria. Amplifications were performed in a 25 μL reaction … WebSep 8, 2015 · PrimeSTAR Max polymerase (2× Master Mix, Takara Bio) was used to generate both altered plasmids. PCR reactions were digested with DpnI for ~2 hours at 37°C and purified with Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel). 1 μl of the purified product (125–150 ng DNA) was combined with 25 μl NEB 5-alpha chemically competent …
WebJun 9, 2024 · Tsingke Biotech leads the way in the development & manufacturing of genomic and biosynthesis products for global researchers. ... 2×T5 Supper PCR … Web2.5 TaqMan real-time RT-PCR assay. The novel PCR assay was developed by employing the 2×T5 Fast qPCR Mix (TSE301, TsingKe Biotech, China) on CFX384 Touch Real-Time PCR …
WebWhen used at the recommended 1X final concentration, the Q5 High-Fidelity Master Mix contains 2 mM Mg ++. Q5 High-Fidelity DNA Polymerase is ... 7,666,645, and …
certified pre owned fort worthWebmy method: 50 ul h20, pic with a yellow tip a E. coli colony, mix with the water, them heat in the PCR machine at 98ºC, for 10 min, them leave at 4ºc for 5 min. Centrifuge 13000g /10 min, use 2 ... certified pre owned grand seikoWeb2、请勿使用dUTP和含有尿嘧啶的引物与模板。 3、PCR Mix应避免反复冻融，短期内多次使用可置于2~8℃保存。使用前，Mix解冻后应充分混匀。 保存条件-25℃~-15℃保存，保质期2年，干冰运输。 本试剂产品仅供科学研究或进一步生产使用，不可用于临床诊断或治疗。 certified pre owned gtr 3000WebqRT-PCR Analysis For qRT-PCR analysis, we extracted RNA using the RNAprep Pure Plant Kit (TIANGEN, Beijing, ... The 2 × T5 Super PCR Mix (Colony) was used. ... certified pre owned grand wagoneerWeb2×T5 Super PCR Mix（Colony）. ¥ 395.00. 货号：TSE005. 规格：. 5×1 mL. 前往科邦邦商城下单. 产品简介. 合成基因组产品及服务. 引物合成. certified pre owned gmc near meWebMoreover, compared to a commercially available kit (2 × T5 Direct PCR kit) (Tsingke, Beijing, China), the electrophoresis results of this colony PCR was also satisfactory with specific … buy us steam accountWeb2×T5 Super PCR Mix （ Colony） 25. μl 172-dicF . 3: μl TY-DicF-56 . 2ul : TY-DicF-60. 2ul : ddH2O . 18: μl PCR process: 98℃ 5min —— 98℃ 10s —— 60℃ 10s —— 72℃ 10s —— Go … buy ust bonds